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一氧化氮合成酶-3（內(nèi)皮型）單克隆抗體

產(chǎn)品貨號(hào)：

BN42006M
中文名稱(chēng)：

一氧化氮合成酶-3（內(nèi)皮型）單克隆抗體
英文名稱(chēng)：

Mouse anti-eNOS Monoclonal antibody
品牌：

Biorigin

貨號(hào)

產(chǎn)品規(guī)格

售價(jià)

備注
BN42006M-50ul

50ul

￥1486.00

交叉反應(yīng)：Human,Mouse,Rat 推薦應(yīng)用：WB,IHC-P,IHC-F,ICC,IF,ELISA
BN42006M-100ul

100ul

￥2360.00

交叉反應(yīng)：Human,Mouse,Rat 推薦應(yīng)用：WB,IHC-P,IHC-F,ICC,IF,ELISA

產(chǎn)品描述

英文名稱(chēng)	eNOS
中文名稱(chēng)	一氧化氮合成酶-3（內(nèi)皮型）單克隆抗體
別名	NOS-3; cNOS; Constitutive NOS; EC NOS; ecNOS; Endothelial nitric oxidase synthase; Endothelial nitric oxide synthase; Endothelial nitric oxide synthase 3; Endothelial NOS; Nitric oxide synthase 3 (endothelial cell); Nitric oxide synthase 3; Nitric oxide synthase 3 endothelial cell; Nitric oxide synthase endothelial; nitric oxide synthase, endothelial; NOS 3; NOS III; NOS type III; NOS3; NOSIII; NOS3_HUMAN.
研究領(lǐng)域	細(xì)胞生物神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 合成與降解內(nèi)皮細(xì)胞
抗體來(lái)源	Mouse
克隆類(lèi)型	Monoclonal
克隆號(hào)	8G1
交叉反應(yīng)	Human, Mouse, Rat,
產(chǎn)品應(yīng)用	WB=1:500-1000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	133kDa
細(xì)胞定位	細(xì)胞漿細(xì)胞膜
性狀	Liquid
濃度	1mg/ml
免疫原	KLH conjugated Synthesised phosphopeptide derived from mouse eNOS around the phosphorylation site of Ser113:
亞型	IgG
純化方法	affinity purified by Protein G
儲(chǔ) 存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed	PubMed
產(chǎn)品介紹	Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function. Function: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. Subunit: Homodimer. Interacts with NOSIP and NOSTRIN. Subcellular Location: Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. Tissue Specificity: Platelets, placenta, liver and kidney. Post-translational modifications: Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity. Phosphorylation of Ser-114 by CDK5 reduces activity. Similarity: Belongs to the NOS family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain. SWISS: P70313 Gene ID: 18127 Database links: Entrez Gene: 287024 Cow Entrez Gene: 4846 Human Entrez Gene: 18127 Mouse Entrez Gene: 24600 Rat Omim: 163729 Human SwissProt: P29473 Cow SwissProt: P29474 Human SwissProt: P70313 Mouse SwissProt: Q62600 Rat Unigene: 647092 Human Unigene: 258415 Mouse Unigene: 44265 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 合成與降解（Synthesis and Degradation) 催化生物體內(nèi)一氧化氮（NO）生成的酶。分神經(jīng)型一氧化氮合成的酶（nNOS or NOS-1）、誘導(dǎo)型一氧化氮合成的酶（iNOS or NOS-2）、內(nèi)皮型一氧化氮合成的酶（eNOS or NOS-3）。此抗體識(shí)別分子量為135kDa的內(nèi)皮型一氧化氮合成的酶。