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磷酸化半胱氨酸蛋白酶9抗體

產(chǎn)品貨號：

BN41064R
中文名稱：

磷酸化半胱氨酸蛋白酶9抗體
英文名稱：

Rabbit anti-Phospho-Caspase-9 (Ser196) Polyclonal antibody
品牌：

Biorigin

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產(chǎn)品規(guī)格

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BN41064R-100ul

100ul

￥2470.00

交叉反應(yīng)：Human,Mouse,Rat 推薦應(yīng)用：WB,IHC-P,IHC-F,ICC,IF,ELISA

產(chǎn)品描述

英文名稱	Phospho-Caspase-9 (Ser196)
中文名稱	磷酸化半胱氨酸蛋白酶9抗體
別名	APAF 3; APAF3; Apoptosis related cysteine peptidase; Apoptotic protease activating factor 3; Apoptotic protease Mch 6; CASP 9; CASP9; Caspase 9 Dominant Negative; Caspase 9 precursor; Caspase 9c; Caspase9; EC 3.4.22.; ICE LAP6; ICE like apoptotic protease 6; MCH6 antibody RNCASP9; CASP9_HUMAN.
產(chǎn)品類型	磷酸化抗體
研究領(lǐng)域	腫瘤免疫學(xué)
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應(yīng)	Human, Mouse, Rat,
產(chǎn)品應(yīng)用	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	35kDa
細(xì)胞定位	細(xì)胞漿
性狀	Liquid
濃度	1mg/ml
免疫原	KLH conjugated Synthesised phosphopeptide derived from human CASP9 around the phosphorylation site of Ser196:FS(p-S)LH
亞型	IgG
純化方法	affinity purified by Protein A
儲存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed	PubMed
產(chǎn)品介紹	Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, BclXL, cIAP1, cIAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf1/cytochrome c complex, and following cleavage by Apaf1, granzyme B, Caspase 3, possibly Caspase 8 and Caspase 10 into large p37 and small p10 subunits. Caspase 9 intereacts with BIRC7 and has been shown to cleave PARP and vimentin. Function: Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts with the inhibitors BIRC2, BIRC4, BIRC5 and BIRC7. Interacts (inactive form) with EFHD2. Interacts with HAX1. Tissue Specificity: Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. Post-translational modifications: Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Similarity: Belongs to the peptidase C14A family. Contains 1 CARD domain. SWISS: P55211 Gene ID: 842 Database links: Entrez Gene: 842 Human Entrez Gene: 12371 Mouse Entrez Gene: 58918 Rat Omim: 602234 Human SwissProt: P55211 Human SwissProt: Q4FJK5 Mouse SwissProt: Q920G4 Rat Unigene: 329502 Human Unigene: 88829 Mouse Unigene: 32199 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Caspase-9半胱氨酸蛋白酶家族成員之一，又稱ICE-Lap6（ICE Like apoptotease 6）參與細(xì)胞凋亡過程和細(xì)胞因子的加工過程，在許多胚胎和成人組織中都有分布。此抗體主要用于腫瘤凋亡的研究。